"How much do your really know about Barack Obama? What does he really believe?" asks the ad, which then cites the failed attack on the Capitol on 9/11, and links it to the Weather Underground attack on the Capitol decades earlier.
The group says it will spend $2.8 million airing the ad in Ohio and Michigan -- which would be the largest single third-party expenditure this cycle.
"Why would Barack Obama be friends with someone who bombed the Capitol and is proud of it?" asks the narrator. "Do you know enough to elect Barack Obama?"
The group, the American Issues Project, is part of a group that isn't required to disclose its donors and is a product of a coalition of conservative groups, including Iowans for Tax Relief. Its president is Ed Martin, a Missouri conservative. Another official, Ed Failor, Jr., is a former McCain aide in Iowa who left after the campaign's shakeup last summer.
The use of 9/11 imagery links Ayers, and Obama, to the American conflict Islamic terror, which is the subject of many viral emails attacking Obama. The group's spokesman, Christian Pinkston, called the suggestion that the group is making any link with Islam "unfair."
"The idea here was to talk about the fact that his friends hate America, and that's who he's aligning himself with," he said.
It's spokesman, Christian Pinkston, is a former aide to presidential candidate Jack Kemp, and went on to run the conservative group Empower America. Pinkston says the ad will launch later this afternoon.